Hotspot

petL-petG

LSC · 63968-64130 · 163 bp

Back to Lupinus

Pi 0.0258

MarkerSeek score 66.10

Primer available yes

Label petL-petG

Feature Metadata

label_name: petL-petG
feature_type: igs
region: LSC
length_bp: 163
variable_sites: 8
pi: 0.0258
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petL-petG_p1	TTCGGTTTTCTACTAGCGGC	CTAACTGATCACCACGTCGG	50.0	55.0	58.0	58.1	344 bp	1.0	81.1
petL-petG_p2	CGGTTTTCTACTAGCGGCTT	CTAACTGATCACCACGTCGG	50.0	55.0	58.0	58.1	342 bp	1.0	81.1
petL-petG_p3	TCGGTTTTCTACTAGCGGCT	CTAACTGATCACCACGTCGG	50.0	55.0	58.8	58.1	343 bp	1.0	74.6
petL-petG_p4	TTCGGTTTTCTACTAGCGGC	TCTAACTGATCACCACGTCG	50.0	50.0	58.0	57.1	345 bp	1.0	74.5
petL-petG_p5	CGGTTTTCTACTAGCGGCTT	TCTAACTGATCACCACGTCG	50.0	50.0	58.0	57.1	343 bp	1.0	74.5