Hotspot

trnH-GUG-psbA

LSC · 122-361 · 240 bp

Back to Lupinus

Pi 0.0425

MarkerSeek score 69.40

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 240
variable_sites: 20
pi: 0.0425
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	CGAGGCTCCATCTATAAATGG	55.0	47.6	58.1	55.7	287-297 bp	1.0	58.5
trnH-GUG-psbA_p2	AATCCACTGCCTTGATCCAC	CGAGGCTCCATCTATAAATGG	50.0	47.6	57.9	55.7	300-310 bp	1.0	58.2
trnH-GUG-psbA_p3	CAATCCACTGCCTTGATCCA	CGAGGCTCCATCTATAAATGG	50.0	47.6	57.9	55.7	301-311 bp	1.0	58.1
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	CGAGGCTCCATCTATAAATGG	50.0	47.6	57.9	55.7	302-312 bp	1.0	58.1
trnH-GUG-psbA_p5	CGCATGGTGGATTCACAATC	CGAGGCTCCATCTATAAATGG	50.0	47.6	57.5	55.7	316-326 bp	1.0	55.1