Hotspot

ycf1

SSC · 123818-129199 · 5382 bp

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Pi 0.01

MarkerSeek score 57.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5382
variable_sites: 54
pi: 0.01
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCTTCTTTTGAATGTCTGCCA	CCATTATTTGGCCCGAGAGA	38.1	50.0	55.7	57.4	294 bp	1.0	49.9
ycf1_p2	TCTTCTTTTGAATGTCTGCCA	TCCATTATTTGGCCCGAGAG	38.1	50.0	55.7	57.4	295 bp	1.0	49.9
ycf1_p3	TCTTCTTTTGAATGTCTGCCAA	CCATTATTTGGCCCGAGAGA	36.4	50.0	56.5	57.4	294 bp	1.0	47.9
ycf1_p4	TCTTCTTTTGAATGTCTGCCAA	TCCATTATTTGGCCCGAGAG	36.4	50.0	56.5	57.4	295 bp	1.0	47.9
ycf1_p5	TTCTTCTTTTGAATGTCTGCCA	CCATTATTTGGCCCGAGAGA	36.4	50.0	56.5	57.4	295 bp	1.0	47.9