Hotspot

trnA-GCA-trnI-AUC

IRa · 134957-135020 · 64 bp

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Pi 0.0156

MarkerSeek score 61.50

Primer available yes

Label trnA-GCA-trnI-AUC

Feature Metadata

label_name: trnA-GCA-trnI-AUC
feature_type: igs
region: IRa
length_bp: 64
variable_sites: 1
pi: 0.0156
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnA-GCA-trnI-AUC_p1	ACAATTAGACACCCAACCCG	TGATTTACTTCACGGGCGAG	50.0	50.0	57.8	58.0	187 bp	1.0	78.8
trnA-GCA-trnI-AUC_p2	CAATTAGACACCCAACCCGT	TGATTTACTTCACGGGCGAG	50.0	50.0	57.8	58.0	186 bp	1.0	78.8
trnA-GCA-trnI-AUC_p3	GCCTGGACAATTAGACACCC	TGATTTACTTCACGGGCGAG	55.0	50.0	58.2	58.0	193 bp	1.0	78.4
trnA-GCA-trnI-AUC_p4	ACAATTAGACACCCAACCCG	GCGAGGTCTCTGGTTCAAAT	50.0	50.0	57.8	57.9	172 bp	1.0	78.1
trnA-GCA-trnI-AUC_p5	CAATTAGACACCCAACCCGT	GCGAGGTCTCTGGTTCAAAT	50.0	50.0	57.8	57.9	171 bp	1.0	78.1