Hotspot

ccsA-ndhD

SSC · 115481-115697 · 217 bp

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Pi 0.023

MarkerSeek score 65.70

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 217
variable_sites: 5
pi: 0.023
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TCTGCAATTGTGGCTTCGAT	AGCAAGTGACAAGGTCGAAT	45.0	45.0	58.2	57.4	374-379 bp	1.0	75.3
ccsA-ndhD_p2	TTCTGCAATTGTGGCTTCGA	AGCAAGTGACAAGGTCGAAT	45.0	45.0	58.4	57.4	375-380 bp	1.0	73.5
ccsA-ndhD_p3	TCTGCAATTGTGGCTTCGAT	GCAAGTGACAAGGTCGAATC	45.0	50.0	58.2	57.1	373-378 bp	1.0	72.6
ccsA-ndhD_p4	ATTCTGCAATTGTGGCTTCG	AGCAAGTGACAAGGTCGAAT	45.0	45.0	57.4	57.4	376-381 bp	1.0	71.5
ccsA-ndhD_p5	TTCTGCAATTGTGGCTTCGA	GCAAGTGACAAGGTCGAATC	45.0	50.0	58.4	57.1	374-379 bp	1.0	70.8