Hotspot

ndhF-rpl32

SSC · 112416-113235 · 820 bp

Back to Lunaria

Pi 0.0209
MarkerSeek score 71.10
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
820
variable_sites
17
pi
0.0209
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
71.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CTGGAAGTGGAATGAAAGGGA CGATGTCCAATACCCCTTCC 47.6 55.0 57.6 57.7 956-959 bp 1.0 68.4
ndhF-rpl32_p2 TTGTTGCTGTCGGAAAAAGT CGATGTCCAATACCCCTTCC 40.0 55.0 56.4 57.7 1004-1007 bp 1.0 67.0
ndhF-rpl32_p3 TGGAAGTGGAATGAAAGGGA CGATGTCCAATACCCCTTCC 45.0 55.0 56.3 57.7 955-958 bp 1.0 66.6
ndhF-rpl32_p4 TTTGTTGCTGTCGGAAAAAGT CGATGTCCAATACCCCTTCC 38.1 55.0 57.1 57.7 1005-1008 bp 1.0 65.0
ndhF-rpl32_p5 CTGGAAGTGGAATGAAAGGGA TCAACGATGTCCAATACCCC 47.6 50.0 57.6 57.3 960-963 bp 1.0 64.9