Hotspot

trnI-AUC-trnA-GCA

IRb · 103352-103415 · 64 bp

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Pi 0.0156
MarkerSeek score 61.50
Primer available yes
Label trnI-AUC-trnA-GCA

Feature Metadata

label_name
trnI-AUC-trnA-GCA
feature_type
igs
region
IRb
length_bp
64
variable_sites
1
pi
0.0156
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnI-AUC-trnA-GCA_p1 TGATTTACTTCACGGGCGAG ACAATTAGACACCCAACCCG 50.0 50.0 58.0 57.8 187 bp 1.0 78.8
trnI-AUC-trnA-GCA_p2 TGATTTACTTCACGGGCGAG CAATTAGACACCCAACCCGT 50.0 50.0 58.0 57.8 186 bp 1.0 78.8
trnI-AUC-trnA-GCA_p3 TGATTTACTTCACGGGCGAG GCCTGGACAATTAGACACCC 50.0 55.0 58.0 58.2 193 bp 1.0 78.4
trnI-AUC-trnA-GCA_p4 GCGAGGTCTCTGGTTCAAAT ACAATTAGACACCCAACCCG 50.0 50.0 57.9 57.8 172 bp 1.0 78.1
trnI-AUC-trnA-GCA_p5 GCGAGGTCTCTGGTTCAAAT CAATTAGACACCCAACCCGT 50.0 50.0 57.9 57.8 171 bp 1.0 78.1