Hotspot

rpl36-rps8

LSC · 79145-79607 · 463 bp

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Pi 0.0195

MarkerSeek score 55.90

Primer available yes

Label rpl36-rps8

Feature Metadata

label_name: rpl36-rps8
feature_type: igs
region: LSC
length_bp: 463
variable_sites: 9
pi: 0.0195
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-rps8_p1	GTTTATGCCTCGGGTTGGAA	TCGGCGGAGAAATTTTGTGT	50.0	45.0	58.2	58.4	594-595 bp	1.0	76.8
rpl36-rps8_p2	TTTATGCCTCGGGTTGGAAC	TCGGCGGAGAAATTTTGTGT	50.0	45.0	58.2	58.4	593-594 bp	1.0	76.8
rpl36-rps8_p3	GTTTATGCCTCGGGTTGGAA	CGGCGGAGAAATTTTGTGTT	50.0	45.0	58.2	57.6	593-594 bp	1.0	76.7
rpl36-rps8_p4	TTTATGCCTCGGGTTGGAAC	CGGCGGAGAAATTTTGTGTT	50.0	45.0	58.2	57.6	592-593 bp	1.0	76.7
rpl36-rps8_p5	TTTTACGAACGGAAGCCCTT	TCGGCGGAGAAATTTTGTGT	45.0	45.0	57.7	58.4	522-523 bp	1.0	76.3