Hotspot

trnP-CCA-psaJ

LSC · 66058-66391 · 334 bp

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Pi 0.0269
MarkerSeek score 65.90
Primer available yes
Label trnP-CCA-psaJ

Feature Metadata

label_name
trnP-CCA-psaJ
feature_type
igs
region
LSC
length_bp
334
variable_sites
9
pi
0.0269
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnP-CCA-psaJ_p1 ACCCGTGACATTTTGTACCC AAACCTGCTAACGAACCGAA 50.0 45.0 58.1 57.8 463 bp 1.0 78.8
trnP-CCA-psaJ_p2 CCCGTGACATTTTGTACCCA AAACCTGCTAACGAACCGAA 50.0 45.0 58.1 57.8 462 bp 1.0 78.8
trnP-CCA-psaJ_p3 ACAGGATTTGAACCCGTGAC AAACCTGCTAACGAACCGAA 50.0 45.0 58.1 57.8 474 bp 1.0 78.7
trnP-CCA-psaJ_p4 CAGGATTTGAACCCGTGACA AAACCTGCTAACGAACCGAA 50.0 45.0 58.1 57.8 473 bp 1.0 78.7
trnP-CCA-psaJ_p5 TGACAGGATTTGAACCCGTG AAACCTGCTAACGAACCGAA 50.0 45.0 58.1 57.8 476 bp 1.0 78.7