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petA-psbJ

LSC · 62025-63052 · 1028 bp

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Pi 0.0119

MarkerSeek score 67.50

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1028
variable_sites: 12
pi: 0.0119
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	GCTGGTATTCTTGTGATCGGT	50.0	47.6	58.0	58.1	1219-1222 bp	1.0	73.0
petA-psbJ_p2	ATCCATTACGTGTCCAAGGC	AGCTGGTATTCTTGTGATCGG	50.0	47.6	58.0	57.8	1220-1223 bp	1.0	72.3
petA-psbJ_p3	CATTACGTGTCCAAGGCCTT	GCTGGTATTCTTGTGATCGGT	50.0	47.6	58.2	58.1	1216-1219 bp	1.0	71.9
petA-psbJ_p4	CATTACGTGTCCAAGGCCTT	AGCTGGTATTCTTGTGATCGG	50.0	47.6	58.2	57.8	1217-1220 bp	1.0	71.2
petA-psbJ_p5	TTACGTGTCCAAGGCCTTTT	GCTGGTATTCTTGTGATCGGT	45.0	47.6	57.6	58.1	1214-1217 bp	1.0	70.5