Hotspot

psaI-ycf4

LSC · 58705-59147 · 443 bp

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Pi 0.00915

MarkerSeek score 61.80

Primer available yes

Label psaI-ycf4

Feature Metadata

label_name: psaI-ycf4
feature_type: igs
region: LSC
length_bp: 443
variable_sites: 4
pi: 0.00915
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-ycf4_p1	GCACTTGCAATGGCTTCTTT	TTGTTTTTCGAGACCCCGTT	45.0	45.0	57.8	58.0	549-555 bp	1.0	79.8
psaI-ycf4_p2	AGTAGGCCTAGTCTTTCCGG	TTGTTTTTCGAGACCCCGTT	55.0	45.0	58.2	58.0	568-574 bp	1.0	79.3
psaI-ycf4_p3	GCACTTGCAATGGCTTCTTT	GGATAAAGGCCCAGCAGAAA	45.0	50.0	57.8	57.9	573-579 bp	1.0	79.0
psaI-ycf4_p4	CCTAGTCTTTCCGGCACTTG	TTGTTTTTCGAGACCCCGTT	55.0	45.0	58.3	58.0	562-568 bp	1.0	78.9
psaI-ycf4_p5	GCACTTGCAATGGCTTCTTT	TAGGATAAAGGCCCAGCAGA	45.0	50.0	57.8	57.8	575-581 bp	1.0	78.6