Hotspot

accD-psaI

LSC · 57947-58590 · 644 bp

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Pi 0.00466

MarkerSeek score 61.40

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 644
variable_sites: 3
pi: 0.00466
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TGGATGCAATTGTACCACGT	AAAGAAGCCATTGCAAGTGC	45.0	45.0	57.8	57.8	813-822 bp	1.0	77.5
accD-psaI_p2	TGGATGCAATTGTACCACGT	CCGGAAAGACTAGGCCTACT	45.0	55.0	57.8	58.2	794-803 bp	1.0	77.0
accD-psaI_p3	TGGATGCAATTGTACCACGT	CAAGTGCCGGAAAGACTAGG	45.0	55.0	57.8	58.3	800-809 bp	1.0	76.6
accD-psaI_p4	TGGATGCAATTGTACCACGT	GCAAGTGCCGGAAAGACTAG	45.0	55.0	57.8	58.9	801-810 bp	1.0	71.4
accD-psaI_p5	TGGATGCAATTGTACCACGT	ATTGCAAGTGCCGGAAAGA	45.0	47.4	57.8	58.0	804-813 bp	1.0	70.5