Hotspot

rpl32-trnL-UAG

SSC · 117948-119191 · 1244 bp

Back to Lumnitzera

Pi 0.00894

MarkerSeek score 51.20

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1244
variable_sites: 11
pi: 0.00894
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 51.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGCGTTGAAAGCTTTTTCGT	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.2	58.0	1367-1372 bp	1.0	80.1
rpl32-trnL-UAG_p2	GGCGTTGAAAGCTTTTTCGT	CAGCGTGTCTACCGATTTCA	45.0	50.0	58.2	58.0	1368-1373 bp	1.0	80.1
rpl32-trnL-UAG_p3	GGCGTTGAAAGCTTTTTCGT	GCTGCATAGCCCAATACCAT	45.0	50.0	58.2	58.1	1969-1974 bp	1.0	79.3
rpl32-trnL-UAG_p4	GGCGTTGAAAGCTTTTTCGT	GAAGTAGCAAAGCCTTGGGT	45.0	50.0	58.2	58.1	1867-1872 bp	1.0	79.3
rpl32-trnL-UAG_p5	GGCGTTGAAAGCTTTTTCGT	TGAAGTAGCAAAGCCTTGGG	45.0	50.0	58.2	58.1	1868-1873 bp	1.0	79.3