Hotspot

rpl14-rps3

LSC · 85141-86877 · 1737 bp

Back to Lumnitzera

Pi 0.00696

MarkerSeek score 62.90

Primer available yes

Label rpl14-rps3

Feature Metadata

label_name: rpl14-rps3
feature_type: igs
region: LSC
length_bp: 1737
variable_sites: 12
pi: 0.00696
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rps3_p1	ATCGGCGATTACTAGCTCCT	GAGGGTAGGGTTCCTCTACA	50.0	55.0	58.1	57.2	1938-1948 bp	1.0	73.5
rpl14-rps3_p2	CCGCTGTTATCCGCTACATT	GAGGGTAGGGTTCCTCTACA	50.0	55.0	58.1	57.2	1888-1898 bp	1.0	73.2
rpl14-rps3_p3	ACATTAATTCTCGAGCCCCG	GAGGGTAGGGTTCCTCTACA	50.0	55.0	57.8	57.2	1905-1915 bp	1.0	72.3
rpl14-rps3_p4	ATCGGCGATTACTAGCTCCT	GAGGGTAGGGTTCCTCTACAA	50.0	52.4	58.1	57.9	1938-1948 bp	1.0	71.4
rpl14-rps3_p5	CCGCTGTTATCCGCTACATT	GAGGGTAGGGTTCCTCTACAA	50.0	52.4	58.1	57.9	1888-1898 bp	1.0	71.0