Hotspot

ndhC-trnV-UAC

LSC · 53497-54995 · 1499 bp

Back to Lumnitzera

Pi 0.00926

MarkerSeek score 67.30

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1499
variable_sites: 13
pi: 0.00926
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GGGGCTAAAACTCCGGAAAT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	1560-1630 bp	1.0	82.0
ndhC-trnV-UAC_p2	GGGGCTAAAACTCCGGAAAT	AAGGCATGTTGGGTCTTTGA	50.0	45.0	57.9	57.6	1627-1697 bp	1.0	80.5
ndhC-trnV-UAC_p3	GGGGCTAAAACTCCGGAAAT	AGGCATGTTGGGTCTTTGAA	50.0	45.0	57.9	57.6	1626-1696 bp	1.0	80.5
ndhC-trnV-UAC_p4	CTGGTCCTTTGCTAATCGGG	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.3	57.8	1577-1647 bp	1.0	80.4
ndhC-trnV-UAC_p5	CCTTTGCTAATCGGGGCTAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.7	57.8	1572-1642 bp	1.0	80.3