Hotspot

ndhK-ndhC

LSC · 53078-53133 · 56 bp

Back to Lumnitzera

Pi 0.0357

MarkerSeek score 73.00

Primer available yes

Label ndhK-ndhC

Feature Metadata

label_name: ndhK-ndhC
feature_type: igs
region: LSC
length_bp: 56
variable_sites: 2
pi: 0.0357
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK-ndhC_p1	AGCGGCCATAGACTAGAGAG	CATGGCGAAAAGGAGCATTG	55.0	50.0	58.1	58.1	198-199 bp	1.0	79.4
ndhK-ndhC_p2	TTTGGGTTGTTCGAGCAAGT	CATGGCGAAAAGGAGCATTG	45.0	50.0	58.2	58.1	131-132 bp	1.0	78.6
ndhK-ndhC_p3	AGCGGCCATAGACTAGAGAG	AAAGGAGCATTGGAGTGGTC	55.0	50.0	58.1	57.8	190-191 bp	1.0	78.4
ndhK-ndhC_p4	GGCCATAGACTAGAGAGCCT	CATGGCGAAAAGGAGCATTG	55.0	50.0	57.7	58.1	195-196 bp	1.0	77.7
ndhK-ndhC_p5	TTTGGGTTGTTCGAGCAAGT	AAAGGAGCATTGGAGTGGTC	45.0	50.0	58.2	57.8	123-124 bp	1.0	77.7