Hotspot

trnC-GCA-ndhJ

LSC · 51020-51808 · 789 bp

Back to Lumnitzera

Pi 0.00887

MarkerSeek score 63.30

Primer available yes

Label trnC-GCA-ndhJ

Feature Metadata

label_name: trnC-GCA-ndhJ
feature_type: igs
region: LSC
length_bp: 789
variable_sites: 7
pi: 0.00887
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	ATCATCCACGACTGAAACGT	55.0	45.0	58.0	57.3	966 bp	1.0	74.6
trnC-GCA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	ATCATCCACGACTGAAACGT	50.0	45.0	58.1	57.3	930 bp	1.0	74.2
trnC-GCA-ndhJ_p3	CTGGTAGAGCAGAGGACTGA	ATCATCCACGACTGAAACGT	55.0	45.0	57.9	57.3	954 bp	1.0	73.5
trnC-GCA-ndhJ_p4	ATGCGTAAGTAATGGTCGGG	ATCATCCACGACTGAAACGT	50.0	45.0	57.8	57.3	983 bp	1.0	72.7
trnC-GCA-ndhJ_p5	GGGATAGCTCAGCTGGTAGA	AATCATCCACGACTGAAACGT	55.0	42.9	58.0	58.0	967 bp	1.0	72.2