Hotspot

trnT-GGU-psbD

LSC · 33744-35012 · 1269 bp

Back to Lumnitzera

Pi 0.01

MarkerSeek score 69.10

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1269
variable_sites: 12
pi: 0.01
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 69.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	GAAACGGTCCCTACGTAACC	55.0	55.0	58.1	58.0	1342-1411 bp	1.0	84.2
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	GAAACGGTCCCTACGTAACC	50.0	55.0	58.1	58.0	1345-1414 bp	1.0	84.0
trnT-GGU-psbD_p3	GGCGTAAGTCATCGGTTCAA	GAAACGGTCCCTACGTAACC	50.0	55.0	58.3	58.0	1319-1388 bp	1.0	82.4
trnT-GGU-psbD_p4	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	55.0	45.0	58.1	58.2	1350-1419 bp	1.0	82.2
trnT-GGU-psbD_p5	GTGGTAGAGTAACGCCATGG	GACCGGACCAACCTACAAAA	55.0	50.0	58.1	57.7	1364-1433 bp	1.0	82.1