Hotspot

trnD-GUC-trnY-GUA

LSC · 32139-32607 · 469 bp

Back to Lumnitzera

Pi 0.0108

MarkerSeek score 63.20

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 469
variable_sites: 5
pi: 0.0108
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	ATTTGATTCCGTCGGGACTG	GACTTGCTTCTTCCTGGGTC	50.0	55.0	58.0	58.2	654-657 bp	1.0	79.5
trnD-GUC-trnY-GUA_p2	ATTTGATTCCGTCGGGACTG	TGGCAATATGTCTACGCTGG	50.0	50.0	58.0	57.8	595-598 bp	1.0	79.2
trnD-GUC-trnY-GUA_p3	ATTTGATTCCGTCGGGACTG	GGCAATATGTCTACGCTGGT	50.0	50.0	58.0	57.8	594-597 bp	1.0	79.2
trnD-GUC-trnY-GUA_p4	ATTTGATTCCGTCGGGACTG	GGGGACGGACTGTAAATTCG	50.0	55.0	58.0	58.4	616-619 bp	1.0	78.3
trnD-GUC-trnY-GUA_p5	ATTTGATTCCGTCGGGACTG	GGGACGGACTGTAAATTCGT	50.0	50.0	58.0	57.6	615-618 bp	1.0	78.1