Hotspot

petN-psbM

LSC · 30382-31280 · 899 bp

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Pi 0.0111
MarkerSeek score 63.40
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
899
variable_sites
10
pi
0.0111
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 TCTTGCTTGGGCTGCTTTAA TGCTACTGCACTCTTCATTCT 45.0 42.9 58.0 57.0 1054-1056 bp 1.0 65.0
petN-psbM_p2 AGTAAGTCTTGCTTGGGCTG TGCTACTGCACTCTTCATTCT 50.0 42.9 57.8 57.0 1060-1062 bp 1.0 63.5
petN-psbM_p3 ATGGGGAAGAAGTGGACTCT TGCTACTGCACTCTTCATTCT 50.0 42.9 57.6 57.0 1000-1002 bp 1.0 62.3
petN-psbM_p4 TCTTGCTTGGGCTGCTTTAA TGCTACTGCACTCTTCATTC 45.0 45.0 58.0 55.5 1054-1056 bp 1.0 60.7
petN-psbM_p5 AGTAAGTCTTGCTTGGGCTG TGCTACTGCACTCTTCATTC 50.0 45.0 57.8 55.5 1060-1062 bp 1.0 59.2