Hotspot

atpH-atpI

LSC · 14635-15873 · 1239 bp

Back to Lumnitzera

Pi 0.0127

MarkerSeek score 57.00

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1239
variable_sites: 15
pi: 0.0127
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AATAGAAGCAAGCCCGACAG	CCTTGAATTCATTCGCGACG	50.0	50.0	58.0	57.9	1766-1825 bp	1.0	82.6
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	CCTTGAATTCATTCGCGACG	45.0	50.0	58.0	57.9	1732-1791 bp	1.0	82.6
atpH-atpI_p3	AATAGAAGCAAGCCCGACAG	TGTTTCGAATTGGTCAGGGG	50.0	50.0	58.0	58.1	1673-1732 bp	1.0	82.6
atpH-atpI_p4	AAGCGAATCGGTATGCCAAT	CCTTGAATTCATTCGCGACG	45.0	50.0	58.0	57.9	2120-2181 bp	1.0	82.1
atpH-atpI_p5	AGCGAATCGGTATGCCAATT	CCTTGAATTCATTCGCGACG	45.0	50.0	58.0	57.9	2119-2180 bp	1.0	82.1