Hotspot

ccsA-psaC

SSC · 112991-113654 · 664 bp

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Pi 0.0267
MarkerSeek score 71.50
Primer available yes
Label ccsA-psaC

Feature Metadata

label_name
ccsA-psaC
feature_type
igs
region
SSC
length_bp
664
variable_sites
40
pi
0.0267
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.94
markerseek_score
71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-psaC_p1 TGGGATCAATCTATTAGGAATCGG CGCAGCATGGGTCTTTCTTA 41.7 50.0 57.7 58.3 740-932 bp 1.0 51.1
ccsA-psaC_p2 GGATCAATCTATTAGGAATCGGGT CGCAGCATGGGTCTTTCTTA 41.7 50.0 57.7 58.3 738-930 bp 1.0 51.1
ccsA-psaC_p3 GGGATCAATCTATTAGGAATCGGG CGCAGCATGGGTCTTTCTTA 45.8 50.0 58.3 58.3 739-931 bp 1.0 50.9
ccsA-psaC_p4 TGGGATCAATCTATTAGGAATCGG ATGAAACAACTCGCAGCATG 41.7 45.0 57.7 57.4 751-943 bp 1.0 48.1
ccsA-psaC_p5 TGGGATCAATCTATTAGGAATCGG CATGAAACAACTCGCAGCAT 41.7 45.0 57.7 57.4 752-944 bp 1.0 48.1