Hotspot

clpP-psbB

LSC · 72602-73246 · 645 bp

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Pi 0.0446
MarkerSeek score 82.40
Primer available yes
Label clpP-psbB

Feature Metadata

label_name
clpP-psbB
feature_type
igs
region
LSC
length_bp
645
variable_sites
60
pi
0.0446
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.98
markerseek_score
82.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
clpP-psbB_p1 CAATGGTTTTATTGTCTTCATCA CGATACCAAGGCAAACCCAT 30.4 50.0 53.6 58.2 628-778 bp 1.0 50.6
clpP-psbB_p2 CAATGGTTTTATTGTCTTCATCA ACACGATACCAAGGCAAACC 30.4 50.0 53.6 58.5 631-781 bp 1.0 50.6
clpP-psbB_p3 CAATGGTTTTATTGTCTTCATCA TGAACACGATACCAAGGCAA 30.4 45.0 53.6 57.4 634-784 bp 1.0 50.6
clpP-psbB_p4 CAATGGTTTTATTGTCTTCATCAT CGATACCAAGGCAAACCCAT 29.2 50.0 54.2 58.2 628-778 bp 1.0 50.6
clpP-psbB_p5 CAATGGTTTTATTGTCTTCATCA ATGAACACGATACCAAGGCA 30.4 45.0 53.6 57.2 635-785 bp 1.0 50.5