Hotspot

psbE-petL

LSC · 64854-66061 · 1208 bp

Back to Luisia

Pi 0.0221
MarkerSeek score 62.70
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1208
variable_sites
49
pi
0.0221
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.90
markerseek_score
62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 CAACCCGCAATGAATAGGGA AAGCGCTCTTAGTTCAGCTC 50.0 50.0 57.9 58.0 1711-1905 bp 1.0 86.2
psbE-petL_p2 CAACCCGCAATGAATAGGGA ATCCGTGAATCTCAATGGCC 50.0 50.0 57.9 58.0 1344-1535 bp 1.0 86.1
psbE-petL_p3 CAACCCGCAATGAATAGGGA ACCGCGCCTGTATTGTAAAT 50.0 45.0 57.9 58.0 1502-1693 bp 1.0 86.0
psbE-petL_p4 ACTGACGAATAACCAACCCG AAGCGCTCTTAGTTCAGCTC 50.0 50.0 57.9 58.0 1724-1918 bp 1.0 85.8
psbE-petL_p5 ACTGACGAATAACCAACCCG ATCCGTGAATCTCAATGGCC 50.0 50.0 57.9 58.0 1357-1548 bp 1.0 85.8