Hotspot

ndhC-trnV-UAC

LSC · 50092-50892 · 801 bp

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Pi 0.0253
MarkerSeek score 59.40
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
801
variable_sites
33
pi
0.0253
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.87
markerseek_score
59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 ATGCTCCTTTCGTCATGCAT TGAATCAGAGCACATGGAGC 45.0 50.0 58.0 58.0 1069-1167 bp 0.666667 71.7
ndhC-trnV-UAC_p2 ATGCTCCTTTCGTCATGCAT CCGAGAAAGTCTACGGTTCG 45.0 55.0 58.0 58.1 895-993 bp 0.666667 71.3
ndhC-trnV-UAC_p3 AATGCTCCTTTCGTCATGCA TGAATCAGAGCACATGGAGC 45.0 50.0 58.2 58.0 1070-1168 bp 0.666667 70.7
ndhC-trnV-UAC_p4 CAATGCTCCTTTCGTCATGC TGAATCAGAGCACATGGAGC 50.0 50.0 57.8 58.0 1071-1169 bp 0.666667 70.6
ndhC-trnV-UAC_p5 ATGCTCCTTTCGTCATGCAT ATCCGGTTCAAGTGCGAATT 45.0 45.0 58.0 58.2 1353-1451 bp 0.666667 70.3