Hotspot

ycf3-trnS-GGA

LSC · 45374-45964 · 591 bp

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Pi 0.00474
MarkerSeek score 58.50
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
591
variable_sites
7
pi
0.00474
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 TTCTCCTGAAGTTGTCGGAA TTCCAATGCTACGCCTTGAA 45.0 45.0 56.4 57.8 723-725 bp 1.0 66.9
ycf3-trnS-GGA_p2 TTTCTCCTGAAGTTGTCGGA TTCCAATGCTACGCCTTGAA 45.0 45.0 56.4 57.8 724-726 bp 1.0 66.9
ycf3-trnS-GGA_p3 TTCTCCTGAAGTTGTCGGAA CGGAGAGAGAGGGATTCGAA 45.0 55.0 56.4 58.3 772-774 bp 1.0 65.8
ycf3-trnS-GGA_p4 TTTCTCCTGAAGTTGTCGGA CGGAGAGAGAGGGATTCGAA 45.0 55.0 56.4 58.3 773-775 bp 1.0 65.8
ycf3-trnS-GGA_p5 TCTCCTGAAGTTGTCGGAAT TTCCAATGCTACGCCTTGAA 45.0 45.0 56.2 57.8 722-724 bp 1.0 65.0