Hotspot

atpH-atpI

LSC · 14435-15082 · 648 bp

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Pi 0.0109
MarkerSeek score 58.20
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
648
variable_sites
17
pi
0.0109
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 TCCGATAGAAGCAAGCCCTA AACTTTGGCTGCGGCTTATA 50.0 45.0 57.9 57.9 766-769 bp 1.0 80.1
atpH-atpI_p2 AGCCAATCCAGCAGCAATAA AACTTTGGCTGCGGCTTATA 45.0 45.0 57.9 57.9 745-748 bp 1.0 79.7
atpH-atpI_p3 CCGATAGAAGCAAGCCCTAC AACTTTGGCTGCGGCTTATA 55.0 45.0 57.8 57.9 765-768 bp 1.0 79.5
atpH-atpI_p4 GCCAATCCAGCAGCAATAAC AACTTTGGCTGCGGCTTATA 50.0 45.0 57.8 57.9 744-747 bp 1.0 79.1
atpH-atpI_p5 CCAGGTCCGATAGAAGCAAG AACTTTGGCTGCGGCTTATA 55.0 45.0 57.8 57.9 771-774 bp 1.0 78.9