Hotspot

trnS-GCU-trnG-UCC

LSC · 8505-9786 · 1282 bp

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Pi 0.021
MarkerSeek score 64.70
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
1282
variable_sites
53
pi
0.021
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.95
markerseek_score
64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA TACCACTAAACTATACCCGC 50.0 45.0 58.0 54.0 1320-1395 bp 1.0 55.0
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC TACCACTAAACTATACCCGC 50.0 45.0 58.0 54.0 1319-1394 bp 1.0 55.0
trnS-GCU-trnG-UCC_p3 CAATCCGACGCTTTAGTCCA TTACCACTAAACTATACCCGC 50.0 42.9 58.0 54.9 1321-1396 bp 1.0 53.9
trnS-GCU-trnG-UCC_p4 AATCCGACGCTTTAGTCCAC TTACCACTAAACTATACCCGC 50.0 42.9 58.0 54.9 1320-1395 bp 1.0 53.9
trnS-GCU-trnG-UCC_p5 CAATCCGACGCTTTAGTCCA TTTACCACTAAACTATACCCGC 50.0 40.9 58.0 55.6 1322-1397 bp 1.0 52.2