Hotspot

psbK-psbI

LSC · 7654-8145 · 492 bp

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Pi 0.0169
MarkerSeek score 59.10
Primer available yes
Label psbK-psbI

Feature Metadata

label_name
psbK-psbI
feature_type
igs
region
LSC
length_bp
492
variable_sites
21
pi
0.0169
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbK-psbI_p1 GTTTGGCAAGCTGCTGTAAG ACGCCCCGGATCATTAGATA 50.0 50.0 57.9 58.1 612-630 bp 1.0 82.1
psbK-psbI_p2 GTTTGGCAAGCTGCTGTAAG TACGCCCCGGATCATTAGAT 50.0 50.0 57.9 58.1 613-631 bp 1.0 82.1
psbK-psbI_p3 GTTTGGCAAGCTGCTGTAAG TTACGCCCCGGATCATTAGA 50.0 50.0 57.9 58.3 614-632 bp 1.0 80.3
psbK-psbI_p4 TGGCAAGCTGCTGTAAGTTT ACGCCCCGGATCATTAGATA 45.0 50.0 58.3 58.1 609-627 bp 1.0 80.1
psbK-psbI_p5 TGGCAAGCTGCTGTAAGTTT TACGCCCCGGATCATTAGAT 45.0 50.0 58.3 58.1 610-628 bp 1.0 80.1