Hotspot

matK-trnK-UUU

LSC · 3256-4424 · 1169 bp

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Pi 0.0196
MarkerSeek score 61.60
Primer available yes
Label matK-trnK-UUU

Feature Metadata

label_name
matK-trnK-UUU
feature_type
igs
region
LSC
length_bp
1169
variable_sites
54
pi
0.0196
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.99
markerseek_score
61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK-trnK-UUU_p1 CCAGATGGATGGGATAGGGT TCCGGGTTGCTAACTCAATG 55.0 50.0 57.9 57.9 1703-1774 bp 1.0 83.0
matK-trnK-UUU_p2 CCAGATGGATGGGATAGGGT ATCCCCATTCCCTATTGGCT 55.0 50.0 57.9 58.1 1798-1869 bp 1.0 82.5
matK-trnK-UUU_p3 CCAGATGGATGGGATAGGGT CGGGTTGCTAACTCAATGGT 55.0 50.0 57.9 58.2 1701-1772 bp 1.0 82.4
matK-trnK-UUU_p4 CCAGATGGATGGGATAGGGT TCGATCCCGAAGAGAAGGAA 55.0 50.0 57.9 57.9 2386-2472 bp 1.0 81.9
matK-trnK-UUU_p5 CCAGATGGATGGGATAGGGT GCTCAACCCACAGAAACTGT 55.0 50.0 57.9 58.3 2277-2363 bp 1.0 80.6