Hotspot

ycf1

SSC · 126504-132095 · 5592 bp

Back to Luffa

Pi 0.34
MarkerSeek score 75.90
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5592
variable_sites
1687
pi
0.34
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.89
markerseek_score
75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 CGAGCCTTGGATCATTCGAT CCTTTTGGTTCTCCCCGAAA 50.0 50.0 57.8 58.0 345 bp 0.5 58.6
ycf1_p2 CGAGCCTTGGATCATTCGAT ACGGTATTCCCAACAATCGG 50.0 50.0 57.8 58.0 1060 bp 0.5 58.5
ycf1_p3 CGAGCCTTGGATCATTCGAT AATTTCCCGAATGGTCCGAG 50.0 50.0 57.8 58.0 608 bp 0.5 58.4
ycf1_p4 CGAGCCTTGGATCATTCGAT CCGAATGGTCCGAGGATTTT 50.0 50.0 57.8 58.0 602 bp 0.5 58.4
ycf1_p5 CGAGCCTTGGATCATTCGAT TACACAGGAAAAGACAGGCG 50.0 50.0 57.8 57.8 832 bp 0.5 57.4