Hotspot

ndhG

SSC · 120808-121338 · 531 bp

Pi 0.475

MarkerSeek score 79.10

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 531
variable_sites: 212
pi: 0.475
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 79.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	ACAATAGCTGGGCCAATAGC	CTCCCCGTTGTTGATTGGAA	50.0	50.0	58.0	58.1	1619 bp	0.5	59.1
ndhG_p2	ACAATAGCTGGGCCAATAGC	ATCTCCCCGTTGTTGATTGG	50.0	50.0	58.0	57.9	1621 bp	0.5	58.9
ndhG_p3	ACAATAGCTGGGCCAATAGC	ACTGCGTTGAATATTGCCCA	50.0	45.0	58.0	58.2	1525 bp	0.5	58.6
ndhG_p4	TGCGATAGGGCCAAGATACT	CTCCCCGTTGTTGATTGGAA	50.0	50.0	58.3	58.1	1798 bp	0.5	57.0
ndhG_p5	ACAATAGCTGGGCCAATAGC	TCCCCGTTGTTGATTGGAAA	50.0	45.0	58.0	57.6	1618 bp	0.5	57.0