Hotspot

ndhA

SSC · 122271-124517 · 2247 bp

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Pi 0.468
MarkerSeek score 79.00
Primer available yes
Label ndhA

Feature Metadata

label_name
ndhA
feature_type
gene
region
SSC
length_bp
2247
variable_sites
886
pi
0.468
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.84
markerseek_score
79.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhA_p1 TGCAGCTCGTACTGTTTGTT CAGACTTGGTGAGATGACGG 45.0 55.0 58.1 58.0 2779 bp 0.5 59.6
ndhA_p2 TTGCAGCTCGTACTGTTTGT CAGACTTGGTGAGATGACGG 45.0 55.0 58.1 58.0 2780 bp 0.5 59.6
ndhA_p3 TGCAGCTCGTACTGTTTGTT ATTGGGGGTTATCAGGACCA 45.0 50.0 58.1 58.0 2921 bp 0.5 59.5
ndhA_p4 TGCAGCTCGTACTGTTTGTT TGGGGGTTATCAGGACCAAT 45.0 50.0 58.1 58.0 2919 bp 0.5 59.5
ndhA_p5 TTGCAGCTCGTACTGTTTGT TGGGGGTTATCAGGACCAAT 45.0 50.0 58.1 58.0 2920 bp 0.5 59.5