Hotspot

ccsA

SSC · 116902-117873 · 972 bp

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Pi 0.468
MarkerSeek score 78.90
Primer available yes
Label ccsA

Feature Metadata

label_name
ccsA
feature_type
gene
region
SSC
length_bp
972
variable_sites
335
pi
0.468
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.74
markerseek_score
78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA_p1 GTGAAATCGGTAGACACGCT GGTATGAGCGGTTTTGTTGC 50.0 50.0 58.0 58.0 1731 bp 0.5 59.8
ccsA_p2 TGAAATCGGTAGACACGCTG GGTATGAGCGGTTTTGTTGC 50.0 50.0 58.0 58.0 1730 bp 0.5 59.7
ccsA_p3 GTGAAATCGGTAGACACGCT TTATCGATGGCTTCCCTTGC 50.0 50.0 58.0 58.0 1758 bp 0.5 59.7
ccsA_p4 TGAAATCGGTAGACACGCTG TTATCGATGGCTTCCCTTGC 50.0 50.0 58.0 58.0 1757 bp 0.5 59.7
ccsA_p5 GTGAAATCGGTAGACACGCT CATGTTTGTGTTCGTCAGGC 50.0 50.0 58.0 58.0 1175 bp 0.5 59.6