Hotspot

rps15-ycf1

SSC · 126077-126503 · 427 bp

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Pi 0.459
MarkerSeek score 76.10
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
427
variable_sites
194
pi
0.459
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
76.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 ATGAGTCGAAGAACACCGTG TTTTATCCCCCAGAGGTCGT 50.0 50.0 57.9 58.0 1200 bp 0.5 59.2
rps15-ycf1_p2 GTAATCGTTGGCGTTTTCCG TTTTATCCCCCAGAGGTCGT 50.0 50.0 58.1 58.0 928 bp 0.5 58.7
rps15-ycf1_p3 ATGAGTCGAAGAACACCGTG CCCAGAGGTCGTAGAGAGTT 50.0 55.0 57.9 57.9 1192 bp 0.5 58.4
rps15-ycf1_p4 CGTTTCTTGTAGCTGGTCCA TTTTATCCCCCAGAGGTCGT 50.0 50.0 57.8 58.0 1139 bp 0.5 58.4
rps15-ycf1_p5 GGTCGTTTCTTGTAGCTGGT TTTTATCCCCCAGAGGTCGT 50.0 50.0 57.8 58.0 1142 bp 0.5 58.4