Hotspot

ndhE-ndhG

SSC · 120580-120807 · 228 bp

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Pi 0.429
MarkerSeek score 75.00
Primer available yes
Label ndhE-ndhG

Feature Metadata

label_name
ndhE-ndhG
feature_type
igs
region
SSC
length_bp
228
variable_sites
85
pi
0.429
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.87
markerseek_score
75.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhE-ndhG_p1 ACAATAGCTGGGCCAATAGC TTTGCCTGGACCGATACATG 50.0 50.0 58.0 58.0 993 bp 0.5 59.6
ndhE-ndhG_p2 ACAATAGCTGGGCCAATAGC TTCGTTGGGACTGGTTCTTG 50.0 50.0 58.0 58.0 888 bp 0.5 59.6
ndhE-ndhG_p3 ACAATAGCTGGGCCAATAGC TAATGGATTTGCCTGGACCG 50.0 50.0 58.0 57.9 1000 bp 0.5 59.5
ndhE-ndhG_p4 ACACTCAAGAAATCCGTCGG TTTGCCTGGACCGATACATG 50.0 50.0 57.9 58.0 1503 bp 0.5 59.1
ndhE-ndhG_p5 ACACTCAAGAAATCCGTCGG TTCGTTGGGACTGGTTCTTG 50.0 50.0 57.9 58.0 1398 bp 0.5 59.1