Hotspot

atpA-atpF

LSC · 12875-12938 · 64 bp

Back to Luffa

Pi 0.0476
MarkerSeek score 79.30
Primer available yes
Label atpA-atpF

Feature Metadata

label_name
atpA-atpF
feature_type
igs
region
LSC
length_bp
64
variable_sites
3
pi
0.0476
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpA-atpF_p1 CCGTCGCCTACTTGAAGTAC ATTGGCATGTTTGGGACGAT 55.0 45.0 58.1 58.1 216-217 bp 1.0 79.2
atpA-atpF_p2 GTCGCCTACTTGAAGTACGG ATTGGCATGTTTGGGACGAT 55.0 45.0 58.1 58.1 214-215 bp 1.0 79.2
atpA-atpF_p3 ACTTGAAGTACGGTACCGGT ATTGGCATGTTTGGGACGAT 50.0 45.0 58.4 58.1 207-208 bp 1.0 76.9
atpA-atpF_p4 TTGCTCAATACGTTCACGGA ATTGGCATGTTTGGGACGAT 45.0 45.0 57.6 58.1 160-161 bp 1.0 76.8
atpA-atpF_p5 GTCGCCTACTTGAAGTACGG TATTGGCATGTTTGGGACGA 55.0 45.0 58.1 57.5 215-216 bp 1.0 76.4