Hotspot

ycf1

SSC · 129497-134824 · 5328 bp

Back to Ludwigia

Pi 0.0354

MarkerSeek score 64.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5328
variable_sites: 417
pi: 0.0354
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCTCTACGCCGTTTAGTGGA	CGAAGGGATTGCAACGACTA	50.0	50.0	58.2	58.0	258-264 bp	0.8	79.2
ycf1_p2	GATTTGCCCAAGGTAGCCTT	CGAAGGGATTGCAACGACTA	50.0	50.0	58.1	58.0	551 bp	0.2	46.8
ycf1_p3	GATTTGCCCAAGGTAGCCTT	TAGCCTTTCTTCGAGGGGAA	50.0	50.0	58.1	58.1	648 bp	0.2	46.4
ycf1_p4	GATTTGCCCAAGGTAGCCTT	GGGGGAGCTAGTTTTTAGGC	50.0	55.0	58.1	57.9	599 bp	0.2	46.4
ycf1_p5	ATGGATTTGCCCAAGGTAGC	CGAAGGGATTGCAACGACTA	50.0	50.0	58.2	58.0	554 bp	0.2	46.2