Hotspot

accD

LSC · 61907-63562 · 1656 bp

Back to Ludwigia

Pi 0.0218

MarkerSeek score 61.20

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1656
variable_sites: 75
pi: 0.0218
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	GTGCTTGTGATCGGATCCAT	TGAATTGCGCATAAGGCAGA	50.0	45.0	58.0	58.3	1831-2003 bp	1.0	85.4
accD_p2	TCCTAGTGCTTGTGATCGGA	TGAATTGCGCATAAGGCAGA	50.0	45.0	58.2	58.3	1836-2008 bp	1.0	84.5
accD_p3	GTGCTTGTGATCGGATCCAT	GCCTAATTTGAATGGCGCAT	50.0	45.0	58.0	57.5	1852-2024 bp	1.0	83.3
accD_p4	GTGCTTGTGATCGGATCCAT	TTGAATTGCGCATAAGGCAG	50.0	45.0	58.0	57.4	1832-2004 bp	1.0	82.9
accD_p5	ATTGTCCTTTTCGGTTCCGT	TGAATTGCGCATAAGGCAGA	45.0	45.0	57.7	58.3	2422 bp	0.2	43.9