Hotspot

petA-psbJ

LSC · 68063-69023 · 961 bp

Back to Ludwigia

Pi 0.0197

MarkerSeek score 66.80

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 961
variable_sites: 45
pi: 0.0197
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGAGAAGGTTCAATTGTCCGA	TTCATACTCCGGGTTAGGCT	42.9	50.0	57.2	57.8	994-1035 bp	1.0	70.8
petA-psbJ_p2	TGAGAAGGTTCAATTGTCCGA	GTTCATACTCCGGGTTAGGC	42.9	55.0	57.2	57.8	995-1036 bp	1.0	70.3
petA-psbJ_p3	TGAGAAGGTTCAATTGTCCGA	TACTCCGGGTTAGGCTCATC	42.9	55.0	57.2	58.3	990-1031 bp	1.0	69.8
petA-psbJ_p4	TGAGAAGGTTCAATTGTCCGA	TCATACTCCGGGTTAGGCTC	42.9	55.0	57.2	58.3	993-1034 bp	1.0	69.8
petA-psbJ_p5	TGAGAAGGTTCAATTGTCCGA	ATACTCCGGGTTAGGCTCAT	42.9	50.0	57.2	57.6	991-1032 bp	1.0	69.0