Hotspot

psbM-trnD-GUC

LSC · 31744-32862 · 1119 bp

Back to Ludwigia

Pi 0.0208

MarkerSeek score 70.20

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1119
variable_sites: 54
pi: 0.0208
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	ACGCAGTCGGAACTAGAATG	TTCAATTGGTCAGAGCACCG	50.0	50.0	57.7	58.5	1222-1249 bp	1.0	80.3
psbM-trnD-GUC_p2	CGCAGTCGGAACTAGAATGA	TTCAATTGGTCAGAGCACCG	50.0	50.0	57.4	58.5	1221-1248 bp	1.0	78.0
psbM-trnD-GUC_p3	ACGCAGTCGGAACTAGAATG	GTTCAATTGGTCAGAGCACC	50.0	50.0	57.7	57.0	1223-1250 bp	1.0	76.1
psbM-trnD-GUC_p4	AAAACGCAGTCGGAACTAGA	TTCAATTGGTCAGAGCACCG	45.0	50.0	57.2	58.5	1225-1252 bp	1.0	76.1
psbM-trnD-GUC_p5	AAACGCAGTCGGAACTAGAA	TTCAATTGGTCAGAGCACCG	45.0	50.0	57.2	58.5	1224-1251 bp	1.0	76.1