Hotspot

trnS-GCU-trnG-UCC

LSC · 9270-10083 · 814 bp

Back to Ludwigia

Pi 0.0184

MarkerSeek score 63.90

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 814
variable_sites: 35
pi: 0.0184
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGACGCTTTAGTCCA	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	877-922 bp	1.0	57.1
trnS-GCU-trnG-UCC_p2	AATCCGACGCTTTAGTCCAC	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	876-921 bp	1.0	57.1
trnS-GCU-trnG-UCC_p3	ACGGAAAGAGAGGGATTCGA	ACGAATCACACTTTTACCACT	50.0	38.1	58.2	55.5	930-975 bp	1.0	55.6
trnS-GCU-trnG-UCC_p4	CAATCCGACGCTTTAGTCCA	AACGAATCACACTTTTACCACT	50.0	36.4	58.0	56.2	878-923 bp	1.0	55.1
trnS-GCU-trnG-UCC_p5	AATCCGACGCTTTAGTCCAC	AACGAATCACACTTTTACCACT	50.0	36.4	58.0	56.2	877-922 bp	1.0	55.1