Hotspot

rps16-trnQ-UUG

LSC · 6466-7923 · 1458 bp

Back to Ludwigia

Pi 0.0257

MarkerSeek score 69.80

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1458
variable_sites: 79
pi: 0.0257
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	GCACGTTGCTTTTTACCACA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	57.8	58.1	871-1526 bp	1.0	86.4
rps16-trnQ-UUG_p2	TCATGTCTGTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	887-1542 bp	1.0	86.2
rps16-trnQ-UUG_p3	AGTCGCACGTTGCTTTTTAC	GAGGTTCGAATCCTTCCGTC	45.0	55.0	57.6	58.1	875-1530 bp	1.0	84.9
rps16-trnQ-UUG_p4	CCACAGCTGATCATGTCTGT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.6	58.1	897-1552 bp	1.0	84.7
rps16-trnQ-UUG_p5	CAAGTCGCACGTTGCTTTTT	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.5	58.1	877-1532 bp	1.0	84.3