Hotspot

psbE-petL

LSC · 66207-67112 · 906 bp

Back to Loxostigma

Pi 0.0198

MarkerSeek score 71.20

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 906
variable_sites: 17
pi: 0.0198
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 71.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	GTCGTATCTTGCTCAGACCA	50.0	50.0	57.8	57.1	972-1021 bp	1.0	76.5
psbE-petL_p2	GTTCTCCTGTGCTTCCAGAC	GTCGTATCTTGCTCAGACCA	55.0	50.0	58.2	57.1	967-1016 bp	1.0	76.2
psbE-petL_p3	TCTCCTGTGCTTCCAGACAT	GTCGTATCTTGCTCAGACCA	50.0	50.0	58.4	57.1	965-1014 bp	1.0	75.0
psbE-petL_p4	AGAACGTTCTCCTGTGCTTC	GTCGTATCTTGCTCAGACCAA	50.0	47.6	57.8	57.8	972-1021 bp	1.0	74.2
psbE-petL_p5	AGAACGTTCTCCTGTGCTTC	AGTCGTATCTTGCTCAGACC	50.0	50.0	57.8	56.8	973-1022 bp	1.0	74.1