Hotspot

ndhC-trnV-UAC

LSC · 51441-52493 · 1053 bp

Back to Loxostigma

Pi 0.0285
MarkerSeek score 63.70
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1053
variable_sites
30
pi
0.0285
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TGCCCAAAAGATATCATATTCGT GCACGTTGGGTCTTTGAAAC 34.8 50.0 56.1 58.2 1186-1201 bp 1.0 42.1
ndhC-trnV-UAC_p2 TGCCCAAAAGATATCATATTCGT AATGCACGTTGGGTCTTTGA 34.8 45.0 56.1 58.3 1189-1204 bp 1.0 42.1
ndhC-trnV-UAC_p3 TGCCCAAAAGATATCATATTCGT ATGCACGTTGGGTCTTTGAA 34.8 45.0 56.1 58.3 1188-1203 bp 1.0 42.1
ndhC-trnV-UAC_p4 TGCCCAAAAGATATCATATTCGT AAATGCACGTTGGGTCTTTG 34.8 45.0 56.1 57.5 1190-1205 bp 1.0 42.1
ndhC-trnV-UAC_p5 TGCCCAAAAGATATCATATTCGT TGCACGTTGGGTCTTTGAAA 34.8 45.0 56.1 58.5 1187-1202 bp 1.0 42.1