Hotspot

trnL-UAA-trnF-GAA

LSC · 48741-49076 · 336 bp

Back to Loxostigma

Pi 0.0298

MarkerSeek score 62.60

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 336
variable_sites: 10
pi: 0.0298
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	ACATGTCAATACCGGCAACA	TCTACCAGCTGAGCTATCCC	45.0	55.0	57.8	58.0	449 bp	1.0	80.2
trnL-UAA-trnF-GAA_p2	ACATGTCAATACCGGCAACA	TTTGAACTGGTGACACGAGG	45.0	50.0	57.8	58.1	485 bp	1.0	79.7
trnL-UAA-trnF-GAA_p3	ACATGTCAATACCGGCAACA	TTAACCATGTGCCAGGAACC	45.0	50.0	57.8	58.1	508 bp	1.0	79.4
trnL-UAA-trnF-GAA_p4	ACATGTCAATACCGGCAACA	GTGCCAGGAACCAGATTTGA	45.0	50.0	57.8	58.1	500 bp	1.0	79.3
trnL-UAA-trnF-GAA_p5	ACATGTCAATACCGGCAACA	TCAGTCCTCTGCTCTACCAG	45.0	55.0	57.8	57.9	461 bp	1.0	79.1