Hotspot

trnD-GUC-trnY-GUA

LSC · 31225-31333 · 109 bp

Back to Loxostigma

Pi 0.055

MarkerSeek score 72.60

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 109
variable_sites: 6
pi: 0.055
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	CGGTGCTCTGACCAATTGAA	GGGGACGGACTGTAAATTCG	50.0	55.0	58.5	58.4	200 bp	1.0	75.8
trnD-GUC-trnY-GUA_p2	CGGTGCTCTGACCAATTGAA	GGGACGGACTGTAAATTCGT	50.0	50.0	58.5	57.6	199 bp	1.0	75.6
trnD-GUC-trnY-GUA_p3	CGGTGCTCTGACCAATTGAA	GATTCTTCCTGGGTCGATGC	50.0	55.0	58.5	58.4	233 bp	1.0	74.9
trnD-GUC-trnY-GUA_p4	CGGTGCTCTGACCAATTGAA	CGGACTGTAAATTCGTTGGC	50.0	50.0	58.5	57.5	195 bp	1.0	74.5
trnD-GUC-trnY-GUA_p5	CGGTGCTCTGACCAATTGAA	GATGCCCGAGTGGTTAATGG	50.0	55.0	58.5	58.7	218 bp	1.0	72.9