Hotspot

petN-psbM

LSC · 29509-30521 · 1013 bp

Back to Loxostigma

Pi 0.029

MarkerSeek score 73.50

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1013
variable_sites: 29
pi: 0.029
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 73.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ACTCGTAGTGTGGGGAAGAA	TCATTCTAGTTCCTACCGCCT	50.0	47.6	58.0	58.0	1109 bp	1.0	74.8
petN-psbM_p2	TCGTAGTGTGGGGAAGAAGT	TCATTCTAGTTCCTACCGCCT	50.0	47.6	58.0	58.0	1107 bp	1.0	74.8
petN-psbM_p3	ACTCGTAGTGTGGGGAAGAA	TCTAGTTCCTACCGCCTTTCT	50.0	47.6	58.0	57.9	1105 bp	1.0	74.2
petN-psbM_p4	TCGTAGTGTGGGGAAGAAGT	TCTAGTTCCTACCGCCTTTCT	50.0	47.6	58.0	57.9	1103 bp	1.0	74.2
petN-psbM_p5	CGTAGTGTGGGGAAGAAGTG	TCATTCTAGTTCCTACCGCCT	55.0	47.6	57.9	58.0	1106 bp	1.0	74.2