Hotspot

psbI-trnS-GCU

LSC · 8483-8598 · 116 bp

Back to Loxostigma

Pi 0.069

MarkerSeek score 70.20

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 116
variable_sites: 8
pi: 0.069
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	AATCCTGGACGTGAAGAATA	TGGACTAAAGCGTCGGATTG	40.0	50.0	53.8	58.0	210 bp	1.0	49.1
psbI-trnS-GCU_p2	ATCCTGGACGTGAAGAATAA	TGGACTAAAGCGTCGGATTG	40.0	50.0	53.8	58.0	209 bp	1.0	49.1
psbI-trnS-GCU_p3	AATCCTGGACGTGAAGAATA	GTGGACTAAAGCGTCGGATT	40.0	50.0	53.8	58.0	211 bp	1.0	49.1
psbI-trnS-GCU_p4	ATCCTGGACGTGAAGAATAA	GTGGACTAAAGCGTCGGATT	40.0	50.0	53.8	58.0	210 bp	1.0	49.1
psbI-trnS-GCU_p5	ATCCTGGACGTGAAGAATAA	ATTGGGAGAGATGGCTGAGT	40.0	50.0	53.8	58.1	228 bp	1.0	48.0