Hotspot

psbK-psbI

LSC · 7918-8371 · 454 bp

Back to Loxostigma

Pi 0.0246

MarkerSeek score 60.50

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 454
variable_sites: 10
pi: 0.0246
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CTTTTGTTTGGCAAGCTGCT	ACGAAGAGTTTCAGAGTAAGCA	45.0	40.9	58.1	57.4	469-512 bp	1.0	64.5
psbK-psbI_p2	GTTTGGCAAGCTGCTGTAAG	ACGAAGAGTTTCAGAGTAAGCA	50.0	40.9	57.9	57.4	464-507 bp	1.0	64.5
psbK-psbI_p3	TGGCAAGCTGCTGTAAGTTT	ACGAAGAGTTTCAGAGTAAGCA	45.0	40.9	58.3	57.4	461-504 bp	1.0	62.5
psbK-psbI_p4	TTGGCAAGCTGCTGTAAGTT	ACGAAGAGTTTCAGAGTAAGCA	45.0	40.9	58.3	57.4	462-505 bp	1.0	62.5
psbK-psbI_p5	TTTGGCAAGCTGCTGTAAGT	ACGAAGAGTTTCAGAGTAAGCA	45.0	40.9	58.3	57.4	463-506 bp	1.0	62.5